ABI 3948 Nucleic Acid Synthesis and Purification System
AVAILABLE FOR IMMEDIATE SHIPMENT
Description
The ABI 3948 Nucleic Acid Synthesis
and Purification System produces
a record 48 purified, 20-mer oligo-nucleotides
in 24 hours without userintervention.
It eliminates most of the
labor involved by automating synthe-
sis, cleavage, deprotection, purifica-
tion, quantitation, and collection,
producing ready-to-use oligos with
typical yields of 2.0 O.D.
Synthesis
The OneStep* columns contain
approximately 50 nmol of 3' nucleo-
side succinate (FastPhoramidite(tm)) for
synthesis. They are color-coded
according to the nucleotide attached.
The ABI 3948 system automatically
dilutes and mixes dry phosphoramidite
monomers with acetonitrile. Synthesis
(detritylation, coupling, capping, and
oxidation) uses a 3.5-minute cycle per
monomer addition.
Cleavage and Deprotection
The ABI 3948 system cleaves the
oligo from the support by delivering
concentrated ammonium hydroxide
for 45 minutes, and then removes the
nucleobase and phosphate protecting
groups by heating at 65 °C for 1 hour.
Purification
The
OneStep column performs a dual role-it provides the 3' nucleoside
succinate support for synthesis, and also contains a polystyrene
medium for purification. The crude, deprotected oligonucleotides
pass through their original OneStep columns where they, ' are
purified by an OPC(r)-type-proce- dure. The 5' DMT group allows
only the correct full-length sequence to be retained by the column.
Failure sequences are washed away and sent to waste. A subsequent
step elutes the purified oligonucleotide in 1 pL of 20 acetonitrile.
Full activity in DNA sequencing and PCR applica- tions is comparable
to the results obtained using HPLC-purified oligos.
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